Vector® Laboratories – TrueVIEW® - New Trial Size!
The Vector® TrueVIEW® Autofluorescence Quenching Kit provides a novel way to diminish unwanted autofluorescence from non-lipofuscin sources and dramatically improve signal-to-noise ratio. It removes unwanted autofluorescence in tissue sections due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin. The quenching action of the kit reagents provides a clear, unambiguous, “true view” localization of the target antigen.
- Effective on problematic tissues such as kidney and spleen
- Easy-to-use, one-step method
- Compatible with a wide selection of fluorophores
- Compatible with standard epifluorescence and confocal laser microscopes
- TrueVIEW with DAPI counterstain available here
One TRIAL kit is sufficient to treat approximately 30 tissue sections.
Trial Kit Components
Kit Contents: 3 mL working solution once mixed
- Vector TrueVIEW Reagent A, 1 mL
- Vector TrueVIEW Reagent B, 1 mL
- Vector TrueVIEW Reagent C, 1 mL
- VECTASHIELD® Vibrance™ Antifade Mounting Medium, 1 mL
Order your Trial Kit:
Why is the TrueVIEW Autofluorescence Quenching reagent applied after completion of the IF assay and not at the start of the procedure?
During product development, applying the TrueVIEW Quenching reagent at the end of our standard IF procedure yielded the most optimal reduction in autofluorescence signal. The reagent is retained on the tissue at the time of mounting, allowing for extended quenching action, with little to no effect on the specific fluorescent signal. However, in applications with very brief staining procedures, such as a primary antibody directly conjugated with a fluorophore, application of TrueVIEW Quenching reagent may be just as effective at the start of the procedure.
Do you have any published references describing the use of TrueVIEW Autofluorescence Quenching Kit?
There are a number of published references describing the use of TrueVIEW Autofluorescence Quenching Kit in the scientific literature. Below are some recent publications: Boucher, J.M., Robich, M., Scott, S.S. et al (2018). Rab27a Regulates Human Perivascular Adipose Progenitor Cell Differentiation. Cardiovasc Drugs Ther., (https://doi.org/10.1007/s10557-018-6813-y) Du, H. et al (2018). A novel mouse model of hemangiopericytoma due to loss of Tsc2. Human Mol. Gen., (https://doi.org/10.1093/hmg/ddy289) Su Y, Hou Y, Wang Q, (2018). The enhanced replication of an S-intact PEDV during coinfection with an S1 NTD-del PEDV in piglets, Veterinary Microbiology, (https://doi.org/10.1016/j.vetmic.2018.11.025) Yoon, J.H., Li, M., Basile, J.R., Lin, Y (2018). Computer-assisted analysis of immunohistological parameters in oral giant cell granulomas, Oral Diseases, (https://doi.org/10.1111/odi.13022)
Is TrueVIEW effective against lipofuscin derived autofluorescence?
The working solution of TrueVIEW works in an electrostatic manner to greatly reduce or eliminate fluorescence in tissue sections induced through the use of an aldehyde based fixative. TrueVIEW is also effective at reducing fluorescence from tissue components such as collagen, elastin and red blood cells. TureVIEW does not work to reduce autofluorescence due to lipofuscin.
How long can I store the TrueVIEW working solution?
Once made up, the working solution of TrueVIEW can be stored either on the bench top or in the fridge (2-8C) for about 48 hours (2 days) without loss of activity or function. Following this time we would suggest discarding unused working solution and make fresh solution as required.
My tissue sections turned blue when I applied TrueVIEW. Is this supposed to happen?
Yes, the working solution is a blue color that does "stain" the tissue section blue. This indicates an active and appropriate chemical reaction is occurring. The blue stain on the section does not fluoresce and does not interfere with the immunofluorescence application.
What mounting media can I use with TrueVIEW?
Both TrueVIEW products, SP-8400 and SP-8500, are supplied with 2 mL of VECTASHIELD Vibrance antifade mounting media. Vector® found that the mounting media does play a crucial role in maintaining the high signal to noise ratio when using TrueVIEW. VECTASHIELD Vibrance is included therefore as a critical component of the kit and it is recommended to use the media supplied for optimal results. At this time we do not have sufficient data to confidently recommend the use of other vendors mounting media with TrueVIEW. Substitution of VECTASHIELD Vibrance with another mounting media may result in less than satisfactory results.
For the wash step after applying the TrueVIEW quenching reagent, can I use buffers other than PBS or detergents?
No, Vector® has found that the TrueVIEW reagent lifts off the tissue using TBS or HEPES buffer. Detergents are incompatible.