Mirus Bio – Transfection

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Mirus offers a range of effective transfection solutions and tools for a comprehensive set of cell lines.  These solutions are intentionally designed
to be gentle on cells and maximize cell delivery while using a simplified, straightforward protocol.


The Transfection Team
TransIT-X2® Dynamic Delivery System
TransIT®-LT1 Transfection Reagent
TransIT®-2020 Transfection Reagent
TransIT®-mRNA Transfection Kit
TransIT®-TKO Transfection Reagent
TransIT®-siQUEST Transfection Reagent
TransIT®-Oligo
 

Electroporation with Ingenio®
Ingenio® EZporator®
Ingenio® Electroporation Solution
 

Cell and Gene Therapy Virus Production
TransIT-VirusGEN® Transfection Reagent
RevIT™ AAV Enhancer

 

 

The Transfection Team

TransIT-X2® Dynamic Delivery System

Mirus TransIT-X2® Delivery SystemA dynamic transfection system for the delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components.

A versatile transfection reagent that is the solution for hard-to-transfection and common cell types. Proven and reliable, TransIT-X2® allows you to avoid guesswork and get it right the first time.

  • Peak performance in a broad range of cell types
  • Suitable for multiple applications
  • Efficient delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 component

  Product Number

   Description   Pkg Size 
  MIR6003   TransIT-X2® Dynamic Delivery System   0.3 mL
  MIR6004   TransIT-X2® Dynamic Delivery System   0.75 mL
  MIR6000   TransIT-X2® Dynamic Delivery System   1.5 mL
  MIR6005   TransIT-X2® Dynamic Delivery System   5 x 1.5 mL
  MIR6006   TransIT-X2® Dynamic Delivery System   10 x 1.5 mL

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TransIT®-LT1 Transfection Reagent

Mirus TransIT-LT1 Transfection ReagentA broad spectrum, low toxicity, DNA transfection reagent.

  • Broad Spectrum DNA Delivery – Utilize one transfection reagent and protocol for a variety of cells
  • Low Cellular Toxicity – Maintain cell density and reduce experimental biases.
  • High Efficiency Delivery – Achieve expression in a large population of cells for experimental success
  • Deliver Single or Multiple Plasmids – Suitable for applications such as virus production

  Product Number

   Description   Pkg Size 
  MIR2304   TransIT®-LT1 Transfection Reagent   0.4 mL
  MIR2300   TransIT®-LT1 Transfection Reagent   1.0 mL
  MIR2305   TransIT®-LT1 Transfection Reagent   5 x 1.0 mL
  MIR2306   TransIT®-LT1 Transfection Reagent   10 x 1.0 mL

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TransIT®-2020 Transfection Reagent

Mirus TransIT-2020 Transfection ReagentA high performance, animal-free, broad spectrum DNA transfection reagent.

  • Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines and primary cells
  • Gentle to Your Cells – Balances high efficiency nucleic acid delivery and low cellular toxicity
  • Animal Origin Free – High performance with maximum compatibility

  Product Number

   Description   Pkg Size 
  MIR5404   TransIT®-2020 Transfection Reagent   0.4 mL
  MIR5400   TransIT®-2020 Transfection Reagent   1.0 mL
  MIR5405   TransIT®-2020 Transfection Reagent   5 x 1.0 mL
  MIR5406   TransIT®-2020 Transfection Reagent   10 x 1.0 mL

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TransIT®-mRNA Transfection Kit

Mirus TransIT-mRNA Transfection KitA high efficiency, low toxicity transfection reagent for large RNA and CRISPR guide RNA.

Each Kit is supplied with the TransIT®-mRNA Transfection Reagent and the mRNA Boost Reagent.

  • Low Cellular Toxicity – Maintain cell density and reduce experimental biases
  • High Efficiency Delivery – Achieve RNA delivery in a large population of cells to ensure experimental success
  • Serum Compatible – Perform transfections in the presence of serum which eliminates the need for a media change and maintains cellular health
  • Deliver Various Sizes of RNA – Ideal for specialized applications, such as virus production,
    protein expression and CRISPR genome editing

  Product Number

   Description   Pkg Size 
  MIR2225   TransIT®-mRNA Transfection Reagent   0.4 mL
  MIR2250   TransIT®-mRNA Transfection Reagent   1.0 mL
  MIR2255   TransIT®-mRNA Transfection Reagent   5 x 1.0 mL
  MIR2256   TransIT®-mRNA Transfection Reagent   10 x 1.0 mL

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TransIT®-TKO Transfection Reagent

A high efficiency, low toxicity, siRNA and plasmid DNA transfection reagent for mammalian cells.

  • Broad Spectrum siRNA and Plasmid DNA Delivery – Utilize one transfection reagent and protocol for a wide variety of cells
  • Low Cellular Toxicity – Maintain cell density and reduce experimental biases
  • Reproducible Results – Obtain consistent, targeted gene knockdowns from day to day
  • High Knockdown Efficiency – Achieve optimal gene silencing in a large percentage of cells to ensure experimental success.

  Product Number

   Description   Pkg Size 
  MIR2154   TransIT®-TKO Transfection Reagent   0.4 mL
  MIR2150   TransIT®-TKO Transfection Reagent   1.0 mL
  MIR2155   TransIT®-TKO Transfection Reagent   5 x 1.0 mL
  MIR2156   TransIT®-TKO Transfection Reagent   10 x 1.0 mL

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TransIT®-siQUEST Transfection Reagent

A high efficiency, low toxicity, siRNA transfection reagent for mammalian cells.

  • Broad Spectrum siRNA Delivery – Utilize one transfection reagent and protocol for a wide variety of cells
  • Low Cellular Toxicity – Maintain cell density and reduce experimental biases
  • Reproducible Results – Obtain consistent, targeted gene knockdowns from day to day
  • High Knockdown Efficiency – Achieve optimal gene silencing in a large percentage of cells to ensure experimental success

  Product Number

   Description   Pkg Size 
  MIR2114   TransIT®-siQUEST Transfection Reagent   0.4 mL
  MIR2110   TransIT®-siQUEST Transfection Reagent   1.0 mL
  MIR2115   TransIT-®siQUEST Transfection Reagent   5 x 1.0 mL
  MIR2116   TransIT®-siQUEST Transfection Reagent   10 x 1.0 mL

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TransIT®-Oligo

A high efficiency, low toxicity, transfection reagent optimized for oligonucleotide delivery into a wide range of cell types.

  • Unique Formulation – Maximize transfection performance of oligonucleotides into a wide range of cells
  • Low Cellular Toxicity – Maintain cell density and reduce experimental biases
  • High Efficiency Delivery – Achieve high transfection efficiency in cells to ensure experimental success

  Product Number

   Description   Pkg Size 
  MIR2164   TransIT®-Oligo Transfection Reagent   0.4 mL
  MIR2160   TransIT®-Oligo Transfection Reagent   1.0 mL
  MIR2165   TransIT®-Oligo Transfection Reagent   5 x 1.0 mL
  MIR2166   TransIT®-Oligo Transfection Reagent   10 x 1.0 mL

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Electroporation with Ingenio®

Mirus Bio offers a full range of electroporation offerings, including; Ingenio® Electroporation Solution, electroporation cuvettes, and the new Ingenio® EZporator® Electroporation System. These products are designed to high efficiency delivery to hard-to-transfect cells without the cost or complication of other systems.

 

Ingenio® EZporator®

Mirus Ingenio® EZporatorThe Ingenio® EZporator® Electroporation System is an easy-to-use electroporation system for mammalian cells.

  • Easily optimize electroporation parameters for each cell type
  • Every system comes with a complimentary Ingenio® Electroporation Kit
  Product Number   Description
  MIR51000   Ingenio® EZporator®

 

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Ingenio® Electroporation Solution

Mirus – Ingenio® Solution, Cell Droppers and Cuvettes

  • Compatible with multiple cell types and devices.
  • Use your existing system including the Ingenio® EZporator®, Nucleofector®, Bio-Rad® or Harvard BTX®
  • Reduce cost when using the Lonza Nucleofector® without sacrificing performance
  • High efficiency electroporation of hard to transfect cells, including primary cells and stem cells
  • Long shelf life - One year shelf life allows maximum usage for your experiments
  • Buy only what you need - Available as solution alone or as part of a kit with cuvettes and cell droppers

  Product Number 

  Description   Pkg Size 
  MIR50125   Ingenio Cell Droppers   50
  MIR50121   Ingenio 0.2 cm Cuvettes   50
  MIR50123   Ingenio 0.4 cm Cuvettes   50
  MIR50112   Ingenio Kit-Amaxa, 0.2 cm Cuvettes/Droppers   25 rxn
  MIR50115   Ingenio Kit-Amaxa, 0.2 cm Cuvettes/Droppers   50 rxn
  MIR50118   Ingenio Kit-Amaxa, 0.2 cm Cuvettes/Droppers   100 rxn
  MIR50113   Ingenio Kit-Other, 0.4 cm Cuvettes/Droppers   25 rxn
  MIR50116   Ingenio Kit-Other, 0.4 cm Cuvettes/Droppers   50 rxn
  MIR50119   Ingenio Kit-Other, 0.4 cm Cuvettes/Droppers   100 rxn

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The Cell and Gene Therapy Virus Production

TransIT-VirusGEN® Transfection Reagent

Mirus - TransIT VirusGEN® Transfection ReagentProven Nucleic Acid Delivery for Large-Scale Virus Production

Designed to enhance delivery of packaging and transfer plasmids to adherent and suspension HEK 293 cell types to increase recombinant adeno-associated virus (AAV) and lentivirus production.

Key benefits of TransIT-VirusGEN® Transfection Reagent include:

  • Performance – Efficient DNA delivery for production of high-titer viral vectors
  • Scalability – Efficient production from < 1 ml to large scale cultures
  • Flexibility – Compatible with different recombinant virus and cell culture systems
  • Animal Origin Free – Fully synthetic transfection reagent formulation

TransIT-VirusGEN® is ideal for academic research and biopharmaceutical discovery. Titers can be further
increased with optimized enhancers included in the VirusGEN® AAV Transfection Kit and VirusGEN® LV
Transfection Kit. For large-scale production of viral vectors, Mirus recommends TransIT-VirusGEN® GMP.

  Product Number

   Description   Pkg Size 
  MIR6703   TransIT-VirusGEN® Transfection Reagent   0.3 mL
  MIR6704   TransIT-VirusGEN® Transfection Reagent   0.75 mL
  MIR6700   TransIT-VirusGEN® Transfection Reagent   1.5 mL
  MIR6705   TransIT-VirusGEN® Transfection Reagent   5 x 1.5 mL
  MIR6706   TransIT-VirusGEN® Transfection Reagent   10 x 1.5 mL

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RevIT™ AAV Enhancer

Mirus - RevIT™ AAV EnhancerPaired with any transfection reagent, the RevIT™ AAV Enhancer delivers higher titers for recombinant adeno-associated virus production.

Produce more patient doses at a lower cost and in less time.

High capacity, high yield, and high-quality virus production are essential for advancing the cell and gene therapy field. To increase the production of viral particles, you can scale up the bioreactor or increase the number of bioreactor runs. In other words, do more of the same process.

An alternative solution is to optimize your transfection process with the VirusGEN® transfection platform and RevIT™ AAV Enhancer. You’ll not only increase productivity, but achieve significant long-term time and cost savings.

This novel enhancer is easily integrated into existing workflows, and it produces 2-4x higher titers across a
range of serotypes, cellular growth media formulations, and transfection platforms. RevIT™ AAV Enhancer
can further drive down upstream manufacturing costs by permitting the use of lower levels of plasmid DNA
while maintaining high-quality titers. Continual improvements in both upstream and downstream AAV
manufacturing processes will allow the next generation of gene therapies to reach a broader audience.

  Product Number

   Description   Pkg Size 
  MIR8000   RevIT™ AAV Enhancer   1.5 mL
  MIR8006   RevIT™ AAV Enhancer   10 x 1.5 mL
  MIR8007   VirusGEN® AAV Kit + RevIT™ for 1L culture   1L Kit
  MIR8008   VirusGEN® AAV Kit + RevIT™ for 10L culture   10L Kit

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