April 2018
Dear Researcher,
We are pleased to present the e-Lynx, a newsletter devoted to keeping our customers up-to-date on new technologies, our new products, suppliers, activities and promotions.
- New Products
- Technical Reports
- Vector Laboratories - Reducing Tissue Autofluorescence: Innovations in Immunofluorescence Analysis Boost Signal-to-Noise Ratios
- Micronic - How to Standardize Tissue Sample Storage
- Associates of Cape Cod – Variability and Test Error with the LAL Assay
- Mirus - Optimization of DNA, RNA and RNP Delivery Methods for Efficient CRISPR/Cas9 Mediated Cell Engineering
- EpiCypher - Nucleosome Remodeling Assay by EpiDyne™-FRET
- Hycult Biotech - Complement in First Episode Psychosis
- Cygnus Technologies - New Methods Help Identify Individual Host Cell Proteins in Final Drug Product
- New Videos
- New Literature from our Sister Company
- Promotions
- MJSBioLynx Well Being
NEW PRODUCTS
NuGEN – Celero™ DNA-Seq Library System
Celero™ DNA-Seq is an innovative library system designed to help researchers streamline library preparation for Illumina sequencers. Celero™ features a fast, easy-to-use, addition-only workflow that eliminates post-ligation bead purification. Also included is NuGEN's breakthrough quantitation method, NuQuant™, that saves both time and cost in measuring library concentration for pooling. With the proven DimerFree technology you can use from 10 ng to 1 μg of intact DNA and greater than 200 ng of FFPE DNA with a single kit without the worry of GC bias.
- Faster library prep with reduced hands-on time: No adaptor or template dilution and no post- ligation purification
- One kit, two workflows: PCR and PCR-free can be performed with the same kit, providing flexibility and reducing the number of kits that need to be purchased and stored
- Simplified library pooling: NuQuant provides molarity without the need for costly and time-consuming methods
Click here for more information.
Click here to order or request a quote.
TECHNICAL REPORTS
Vector Laboratories - Reducing Tissue Autofluorescence: Innovations in Immunofluorescence Analysis Boost Signal-to-Noise Ratios
Immunofluorescence can provide clear subcellular visualization of proteins, glycans, and small biological and nonbiological molecules. However, tissue autofluorescence often impairs or prevents the effective use of this tool. As will be described, a new technology has been developed that significantly lowers autofluorescence and dramatically enhances signal-to-noise ratios.
Click here to read more. (Link to Genetic Engineering & Biotechnology News website)
Micronic - How to Standardize Tissue Sample Storage
The validity of the data of a research project depends on the quality of the tissue samples. Therefore, it’s important that a tissue storage facility preserves the quality of its samples. Variations in tissue sample storage conditions will lead to variations in the quality and characteristics of the tissue samples. To prevent quality alterations, strict standards in tissue banking are needed. Understanding the potential of the research on tissue samples leads to the conclusion that the standardization of biobanking practices are of utmost significance.
Click here to read more. (Link to Micronic website)
Associates of Cape Cod – Variability and Test Error with the LAL Assay
American Pharmaceutical Review™ article re-publication.
The Limulus Amebocyte Lysate (LAL) assay is the compendial test for the examination of bacterial endotoxin in pharmaceutical products (as described in USP chapter <85>), in-process material, and pharmaceutical grade water.
With any biological tests, measurements are susceptible to variations in analytical conditions. Here the LAL assay has a relatively high level of variability even for a biological assay. This variation derives from 3 principle sources: reagents, product, and method. This paper examines some of the reasons for LAL test variation, focusing on photometric methods (chromogenic and turbidimetric), and considers how variation can be assessed through good laboratory quality control.
Click here to read more.
Mirus - Optimization of DNA, RNA and RNP Delivery Methods for Efficient CRISPR/Cas9 Mediated Cell Engineering
The success of CRISPR genome editing experiments is limited by the intracellular delivery and expression of Cas9 Protein and gRNA. Many methods for achieving CRISPR mediated cleavage have been identified and the choice of DNA, RNA or ribonucleoprotein (RNP) format is dictated by experimental goal and cell type. Transfection of each type of molecule requires specific considerations for efficient functional delivery. We performed transfections using different combinations of molecules including: plasmid DNA, messenger RNA, Cas9 protein and gRNA to maximize targeting of the Cyclophilin B (PPIB) gene in HEK293 T/17 and other mammalian cell types. Their results extend the utility of the CRISPR/Cas9 system by identifying optimal transfection conditions for intracellular delivery of Cas9 and gRNA in different formats.
Click here to read more.
EpiCypher - Nucleosome Remodeling Assay by EpiDyne™-FRET
Chromatin remodeling, or the repositioning of nucleosomes, regulates DNA access and thus gene expression and genome repair. Many ATP-dependent remodeling enzyme complexes are associated with human disease but are challenging study targets due to the requirement for nucleosome-based substrates. EpiCypher has addressed this need by developing the EpiDyne platform of fully recombinant remodeling substrates to monitor nucleosome repositioning along DNA. Here, we demonstrate the utility of EpiDyne-FRET in a homogeneous remodeling assay using Fluorescence Resonance Energy Transfer (FRET) readout.
Click here to read more.
Hycult Biotech - Complement in First Episode Psychosis
There has been debate over the past few years about the criteria currently used to classify patients with psychiatric disorders. The lack of objective and quantitative diagnostic criteria means that we rely on symptom-based categorization when deciding on patient classification and less on the underlying biology. Accumulating evidence suggests that inflammation and the immune system is the cause of such psychiatric disorders. The complement system is one such system, particularly the classical pathway, that potentially could be an important contributor.
Click here to read more. (Link to Hycult Biotech website)
Cygnus Technologies - New Methods Help Identify Individual Host Cell Proteins in Final Drug Product
Impurities can negatively affect the stability, safety, and efficacy of protein therapeutics. Host cell proteins are a major part of process-related impurities during biologics production, and therefore must be carefully monitored and controlled. With Host Cell Protein ELISA being a critical component of ensuring process consistency and product purity, regulatory agencies have measures in place to ensure the Host Cell Protein ELISA used by a sponsor is fit for this purpose. BioPharm International recently spoke with Ken Hoffman, president of Cygnus Technologies, about best practices and new technologies for host cell protein detection and identification.
Click here to read more.
NEW VIDEOS
Hycult Biotech - RELENT: For a Safer and More Effective Treatment of Patients Who Suffer From Autoimmunity
Hycult Biotech is always eager to participate in research projects and to be the connection between science and industry by jointly developing tools for monitoring inflammatory and autoimmune diseases. As a participant in the RELENT (RELapses prevENTion in chronic autoimmune disease) consortium, Hycult Biotech has developed immunoassays that help monitor autoimmune disease and allow better treatment of these patients. More information about the RELENT consortium and what it stands for can be found in the video.
Click here to watch. (Link to Hycult Biotech website)
MiTeGen’s patented family of MicroMounts and MicroLoops are manufactured from a highly x-ray transparent polymer into precise shapes and sizes using cutting-edge photolithography techniques. These x-ray transparent loops can go directly into the beam, so users select a loop size that is slightly smaller than the crystal. They then easily harvest the crystal, which sits directly onto the surface of the loop. This reduces unwanted fluid around the crystal, leading to easier cryoprotecting, faster cryocooling, and less ice formation.
Click here to watch. (Link to YouTube)
Biosensing Instrument - Surface Plasmon Resonance Microscopy
This video introduces the Surface Plasmon Resonance Microscopy technology from Biosensing Instrument, and how it is used to detect small molecules and their interaction with membrane protein in vitro.
Click here to watch. (Link to YouTube)
NEW LITERATURE FROM OUR SISTER COMPANY
Knauer – Azura Bio Purification Brochure
Complete solutions for FPLC on a minimum footprint: AZURA FPLC systems combine flexibility and reliability for protein purification.
Click here to download.
Contact our Customer Service Team to request a printed copy of this publication.
Knauer products are available to us through our Sister Company, Chromatographic Specialties Inc.
To simplify your purchasing process, Chromatographic Specialties Products can be purchased through MJSBioLynx, and vice versa.
PROMOTIONS
Click here
|
Vector Laboratories - Buy a Vector® TrueVIEW™ Autofluorescence Quenching Kit and Get 50% OFF a VECTASHIELD® Antifade Mounting Medium
Vector® TrueVIEW™ Quencher uses a unique, patent-pending quenching agent to specifically bind and quench autofluorescent elements from non-lipofuscin sources, even in the most problematic tissues.
VECTASHIELD® Antifade Mounting Media formulations offer unsurpassed protection against fading and photobleaching, thereby, providing excellent signal retention for image acquisition and specimen archiving.
Click here for more information on Vector® TrueVIEW™
Click here for more information on VECTASHIELD®
Click here to order or request a quote.
Offer valid until June 30th, 2018.
Cannot be combined with other offers or discounts.
Promotion Code VHS18.
Micronic – Buy One, Get One Specials on Cappers, Tubes and Scanners
These offers will help improve your workflow by automating the process of (de)capping and scanning your sample storage tubes.
Buy One Manual Capper CM480, Get One at 50% OFF
The CM480 Capper offers a cost effective way of applying uniform pressure to push caps in an entire 96 tubes rack in only a single action.
Click here to order.
Buy One Screw Cap Recapper SR008, Get One at 50% OFF
The Recapper SR008 handheld device offers users the ability to cap, decap or recap a row of 8 tubes in less than 5 seconds! This system minimizes sample evaporation, significantly improves productivity, and ensures each screw cap is tightened securely and uniformly.
Click here to order.
Buy One Case of External Thread Tubes, Get One Wireless Scanner for FREE
The small lightweight scanner is practical to carry around, easy-to-use and can read any 1D barcode or 2D Data-Matrix code on 24-, 48- and 96-well format tubes and racks.
Click here to order.
Offers Valid until June 30th, 2018.
Cannot be combined with other offers or discounts.
Promotion Code MIC311
How Mindfulness Improved NB Power’s Safety Record
"An electrical utility, NB Power, has adopted the use of mindfulness as part of its corporate health and safety psychological health strategy. Recognizing that the increasing pressure of responding to a changing work environment as well as providing customer care oft"en left employees in a less than clear mindset, the concept of mindfulness as part of the safety strategy was proposed – and eventually accepted.
Click here to read more. (Link to the Globe and Mail website)
Click here to visit the MJSBioLynx Well Being website.