February 2019
Dear Researcher,
We are pleased to present the e-Lynx, a newsletter devoted to keeping our customers up-to-date on new technologies, our new products, suppliers, activities and promotions.
- PROMOTIONS
- USA Scientific - FREE Pizza Party Promotion
- Micronic – FREE Gifts with Purchase of Barcode Readers, Hybrid Tubes and Cappers
- Vector Laboratories – Save 20% OFF VECTASHIELD® Vibrance™ Antifade Mounting Media
- Mirus Bio - Buy One Ingenio® Electroporation Kit, Get One FREE when you Purchase an Ingenio® EZporator® System
- NEW PRODUCTS
- Vector Laboratories – TrueVIEW™ Autofluorescence Quenching Kit Now with VECTASHIELD® Vibrance™
- Micronic – Rack Reader DR500 Line
- Tecan Genomics (NuGEN) – Celero PCR Workflow with Enzymatic Fragmentation DNA-Seq Kit
- abc biopply – 3D CoSeedis™ Cell Co-Culture System
- Labnet – Enduro™ GDS II Gel Documentation System
- NEW LITERATURE
- TECHNICAL REPORTS
- ASSOCIATES OF CAPE COD INCORPORATED™ - Removal of Endotoxin from Protein in Pharmaceutical Processes
- abc biopply - Extracellular Vesicles Composition Affected by 3D Cell Architecture
- Hycult Biotech - sMR: Predictor of disease severity CAP?
- EpiCypher – EpiDyne®: Exploring Chromatin Remodeling Substrates with Nucleosomes
- ANNOUNCEMENTS
- MJSBioLynx WELL BEING
PROMOTIONS
USA Scientific - FREE Pizza Party Promotion
It's back!
For the cold Canadian winter months of January to March 2019, enjoy hot, delicious pizza on us!
Accumulate $500 in Purchases of USA Scientific Products from MJSBioLynx and Receive a $50 Pizza Gift Certificate*.
USA Scientific offers cell culture labware, pipets and tips, plates, tubes and much more.
Click here to order or to request a quote
Offer valid until March 31st, 2019.
Please reference Promotion Code US2019PZA.
$500 order value is after any applicable discounts or coupons and before taxes and shipping charges.
Limit: Two pizza parties per lab; Cannot be combined with other offers.
* Please note that if you are unable to accept our pizza gift we would be pleased to offer you a $50 voucher towards your next order of $100 or more of USA Scientific products (Coupon applied after taxes and shipping). Coupons are valid until April 30th, 2019.
Micronic – FREE Gifts with Purchase of Barcode Readers, Hybrid Tubes and Cappers
Buy One Rack Reader DR500 or DR505, Get One FREE Side Barcode Reader
The Rack Reader DR500 and DR505 are small benchtop solutions that can read 2D codes quickly and cost effectively within seconds. The compact readers are built for high throughput and automated systems. The DR505 version has an anti-frost system, which enables users to read racks filled with 2D coded tubes straight from the freezer.
Click here to order or request a quote.
Buy 2 Cases of Hybrid Tubes with External Threads, Get 5 FREE Tube Selector Tools
Hybrid tubes offer a unique combination of 4 coding concepts on one tube for automated and visual sample identification. The tubes feature external threads for minimized cross-contamination, thick tube walls for storage in ultra-low temperatures, and production in certified class-7 clean rooms.
This offer applies to 0.75 and 1.40 mL racked hybrid tubes.
Click here to order or request a quote.
Buy One Push Cap Capper CM480, Get One FREE manual Decapper
The CM480 Capper offers a cost effective way of applying uniform pressure to push caps in an entire 96 tube rack in only a single action.
Click here to order or request a quote.
Offers valid until March 31st, 2019.
Promotion code MS0219.
Cannot be combined with other offers.
Vector Laboratories – Save 20% OFF VECTASHIELD® Vibrance™ Antifade Mounting Media
VECTASHIELD™ Vibrance™ prevents rapid photobleaching of fluorescent proteins and fluorescent dyes on cell and tissue section preparations. It is supplied as an aqueous, ready-to-use formulation which will cure (set) over several hours at room temperature to immobilize the coverslip. No further sealing of the coverslip is required.
Click here for more information.
Click here to order or request a quote.
Offer valid until February 28th, 2019.
Promotion Code VECT5218.
Cannot be combined with other offers or discounts.
Mirus Bio - Buy One Ingenio® Electroporation Solution, Get One FREE with Purchase of an Ingenio® EZporator® System
Ingenio® is a broad spectrum solution that supports high efficiency electroporation with minimal toxicity and replaces standard electroporation solutions including phosphate buffered saline and serum-free media.
Purchase the new Ingenio® EZporator® System and take advantage of our Buy One Get One FREE offer on your first purchase of the Ingenio® Electroporation Solution.
Click here to order or request a quote.
Promotion Code MIREZ19.
Offer valid on first purchase of the Ingenio® Solution or Kit after purchase of the Ingenio® EZporator® System.
Free product must be the same product number as the purchased product.
Promotion is subject to change.
NEW PRODUCTS
Vector Laboratories – TrueVIEW™ Autofluorescence Quenching Kit Now with VECTASHIELD® Vibrance™
TrueVIEW™ Autofluorescence Quenching Kit uses a unique, patent pending quenching agent to specifically bind and quench autofluorescent elements from non-lipofuscin sources, even in the most problematic tissues. TrueVIEW™ provides a specific reduction in autofluorescence and improved signal to noise ratios.
VECTASHIELD™ Vibrance™ prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. It is supplied as a ready-to-use formulation which will cure (set) over several hours at room temperature to immobilize the coverslip. No further sealing of the coverslip is required.
Click here for more information.
Click here to order or request a quote.
Micronic – Rack Reader DR500 Line
The Micronic Rack Reader DR500 and DR505 are small benchtop solutions that can read 2D codes quickly and cost effectively within seconds. The compact readers are built for high throughput and automated systems. The DR505 version has an anti-frost system, which enables users to read racks filled with 2D codes tubes straight from the freezer. All Micronic Rack Readers are supplied with Micronic Code Reader Software to ensure an optimal performance.
Click here for more information.
Click here to order or request a quote.
Tecan Genomics (NuGEN) – Celero PCR Workflow with Enzymatic Fragmentation DNA-Seq Kit
A simple and robust solution for DNA sequencing on Illumina sequencers
This library preparation kit is integrated with NuQuant, a proprietary library quantification method for efficient and accurate quantitation of Next-Generation Sequencing (NGS) libraries. The NuQuant method eliminates the need for time-consuming or inaccurate library quantification methods like qPCR, fluorometry (e.g. Qubit®) or microfluidic electrophoresis (e.g. Bioanalyzer®) allowing DNA-Seq libraries to be constructed and quantified in a single day.
- Complete DNA-Seq kit with enzymatic fragmentation that comes standard with all reagents for library preparation for Illumina sequencers
- One bead purification step in the entire library preparation workflow
- Broad dynamic input range from 10 ng - 500 ng
- NuQuant library quantification method integrated at no additional cost to accurately quantify library concentrations (in nM)
- Eliminate adaptor dimers without adaptor titration regardless of sample input using the DimerFree™ technology
- Address Illumina "index hopping" and unique barcodes for each sample with a standard pre-plated UDI adaptor plate
Click here for more information. (Link to NuGEN website)
Click here to order or request a quote.
abc biopply – 3D CoSeedis™ Cell Co-Culture System
3D CoSeedis™ is a novel scaffold-free 3D cell co-culture system. It consists of a unique conical agarose matrix array (CAMA) that allows the formation of spheroidal and non-spheroidal cell aggregates in a highly-reproducible and consistent manner. The conically shaped microwells within the array allow precise determination of aggregate volume and cell growth. Furthermore, the modular composition of the 3D system allows distance co-cultures and, consequently, the standardisation of protocols.
The 3D CoSeedis™ system broadens the range of cells accessible to 3D evaluation substantially. Furthermore, it provides validated and standardised workflows and therefore takes 3D cell technologies to a new level of reliability.
Image: A) Scan of a single 3D CoSeedis™ matrix containing ca. 900 spheroids for volumetric analysis; B) Electron microscopic image of 3D CoSeedis™ surface showing microwells.
Click here for more information.
Click here to order or request a quote.
Labnet – Enduro™ GDS II Gel Documentation System
The GDS II comes in either a 302nm or a 365nm version and runs on a Windows based PC (not included). The GDS Touch II comes in either a 302nm or 365nm wavelength version with 470nm epi-blue lights for use with “Safe” Dyes that eliminate need for ethidium bromide. Both versions now come with a storage location on its top to help maximize bench space and provide a place for accessories so you know where to look.
- Designed to be user friendly
- No focusing
- Auto-exposure works even with faint bands
Click here for more information.
Click here to order or request a quote.
NEW LITERATURE
Matreya – January 2019 Newsletter for Glyco/Sphingolipid Research
In this issue: Interactions of ceramides and proteins; Sulfatides in metachromatic leukodystrophy
Click here to read more.
TECHNICAL REPORTS
ASSOCIATES OF CAPE COD INCORPORATED™ - Removal of Endotoxin from Protein in Pharmaceutical Processes
American Pharmaceutical Review™ article re-publication. 
Bacterial endotoxin is the lipopolysaccharide component of the cell wall of gram-negative bacteria, together with other cellular material that combines to form an endotoxin complex. Endotoxin is pyrogenic and it presents a risk to patients who are administered intravenous and intramuscular preparations. Thus, bacterial endotoxins pose a risk to many pharmaceutical processes and, where not controlled, to the finished products. There are different methods for endotoxin removal. These include depyrogenation, such as dry-heat processes applied to glassware, and rinsing, as might be applied to closures. These areas receive reasonable coverage within the pharmaceutical sector. Areas that are actively discussed within biotechnology fields but which receive less attention in the wider pharmaceutical context are steps to remove endotoxin bound to protein. This article considers some of the biotechnological applications for endotoxin removal.
Click here to read more.
abc biopply - Extracellular Vesicles Composition Affected by 3D Cell Architecture
3D CoSeedis™ has recently been used to demonstrate the effect of 3D cellular architecture on microRNA and protein cargo of extracellular vesicles. In a recent study, 3D CoSeedis™ was used to address the impact of cellular architecture on extracellular vesicle content and function. To do so, the authors used comparative analysis of biochemical features, RNA and proteomic profiles of vesicles secreted by both, 2D and 3D cultures of gastric cancer cells.
Click here to read more. (Link to abc biopply website)
Hycult Biotech - sMR: Predictor of disease severity CAP?
Loonen, A et al; Soluble mannose receptor levels in blood correlate to disease severity in patients with community-acquired pneumonia. Immunology Letters 2018.
Community-acquired pneumonia (CAP) is the most common form of pneumonia and is a leading infectious cause worldwide. sMR, soluble mannose receptor, is a new serum marker for macrophage activation. Its extracellular domains are able to bind several ligands, like mannose, collagen and sulfated carbohydrates. Studies have shown that certain infection markers are (partly) capable to predict disease development in CAP. Since the need for new biomarkers to predict disease prognosis more precisely is still present, sMR was suggested. Results show that patients with sMR levels between 100 - 430 ng/ml had a 22.7% chance to develop severe disease, while patients with higher levels had a 33.3% chance.
Click here for more information on the Soluble Mannose Receptor ELISA Kit.
EpiCypher – EpiDyne®: Exploring Chromatin Remodeling Substrates with Nucleosomes
Nucleosomes are the fundamental repeating units of chromatin, comprised of a histone octamer wrapped by ~147bp DNA. A common focus of epigenetics research is the addition / removal of various post-translational modifications (PTMs) to histone tails, and how these marks impact downstream processes, such as transcription, cell division, and disease. Equally important, but less well understood, are the complexes that assemble, reformat, slide or eject nucleosomes in existing chromatin architecture. These nucleosome remodeling complexes are large, multi-protein structures, which are powered by the hydrolytic activity of associated ATPases1. Nucleosome remodeling is essential to the regulation of multiple pathways in cells, including transcription, genome replication, and DNA damage repair.
Click here to read more. (Link to EpiCypher website)
ANNOUNCEMENTS
Vector Laboratories – VECTASTAIN® ABC Packaging Change
VECTASTAIN ABC Kit reagents will soon be supplied in smaller boxes with a new design. The large volume, empty mixing bottles will no longer be provided in the kit format, thereby allowing for a reduction in box size, saving you fridge space and fewer components to recycle.
For labs that use the large volume reagent mixing bottles, they will continue to be offered as a pack of 3 utility dropper bottles, available as a separate item for purchase.
Click here for more information.
Click here to order or request a quote for the dropper bottles.
NuGEN is Now Tecan Genomics
We are proud to announce that NuGEN Technologies has joined the Tecan Group, and is now officially known as Tecan Genomics. This acquisition offers real value to their customers and their research, adding expertise in automation to their existing NGS reagent knowledge, and will contribute to the development of combined solutions for every lab.
Their commitment to serving customers in the best way possible remains constant, and their website www.nugen.com will remain unchanged for the time being.
Click here for more information. (Link to Tecan Genomics (NuGEN) website)
MJSBioLynx WELL BEING
Taking a Walk Will Boost Your Creativity and Problem-Solving
“Tchaikovsky took a walk every morning, before sitting down to work on his music. Einstein walked on the beach when he needed to work out complex problems. Steve Jobs preferred to conduct meetings while walking.
These and many more of the greatest minds in history made walking a key part of the fabric of their lives and work. In 2014, a study out of Stanford produced some truly wonderful results that may help to explain why. Their findings support the role of walking as a path to brilliant ideas and prodigious creativity.”
Click here to read more. (Link to Psychology Today website)
Click here to visit the MJSBioLynx Well Being website.