E-Lynx Newsletter

June 2018

 

Dear Researcher,

We are pleased to present the e-Lynx, a newsletter devoted to keeping our customers up-to-date on new technologies, our new products, suppliers, activities and promotions.

 

 

 

NEW PRODUCTS

 

Micronic Screw Cap Recapper CS500, CS510, and CS520

Developed in conjunction with several leading laboratories, these Screw Cap Recappers offer the ability to cap, decap or recap a row of tubes in less than 5 seconds and a whole rack in only 1 minute.  This is 10 times faster than by hand and it helps to improves productivity.

  • Cost-effective solution
  • Each screw cap is reproducibly tightened securely and uniformly for an optimal sealing performance
  • Cycle time: caps a row of 8, 6 or 4 tubes in 4 seconds and a whole rack of 96, 48 or 24 tubes within 45-60 seconds
  • No need for battery recharging, available to use once plugged in

 

Click here for more information.
Click here to order or request a quote.

 

EpiCypher - EpiTriton™ Histone Peptide Array

Designed for rapid and high-throughput screening of effector protein, antibody and enzyme interactions with a comprehensive library of combinatorially-modified and biotinylated histone peptides immobilized on a streptavidin-coated glass slide. The peptides encompass over 296 unique modifications on the four core histones (H2A, H2B, H3 and H4) and several histone variants.  Every Array contains more than 292 histone peptides spotted 12 times each for high quality detection and analysis of antibody or protein binding or enzyme activity.

  • Examine the selectivity and specificity of histone modification antibodies
  • Analyze the specificity of histone binding proteins
  • Identify substrates for histone-modifying enzymes

 

Click here for more information. (Link to EpiCypher website)
Click here to order or request a quote. 

 

Heathrow Scientific – Mini Vortexer

  • Made from durable ABS and silicone materials to resist most commonly used chemicals
  • Can be used with tubes ranging from 2 mL to 50 mL
  • Low profile design and small footprint (only 4.3” wide) save valuable space on a bench top and in fume hoods
  • Rapid vortex action saves time and enables quick mixing of samples
  • Easy one-handed touch start operation and shaking stops when the tube is lifted
  • Maintenance free design and 3 year warranty help reduce the cost of ownership
  • Quiet operation and low vibration on the vortex machine helps users stay focused

 

Click here for more information.
Click here to order or request a quote.

 

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PROMOTIONS

Click here
to see all our current promotions

 

 

USA Scientific - Buy 3, Get 1 FREE on TipOne® Pipette Tips

  • Excellent Quality at an Affordable Price
  • Secure, low force fit and accurate delivery
  • No clarifiers or processing additives  
  • Certified free of detectable RNase, DNase, DNA, and pyrogens
  • Refillable system saves storage, reduces waste, and is easy to load

For a limited time, Buy any 3 packs of TipOne® Pipette Tips and Get 1 Pack FREE!

Click here for more information.
Click here to order or request a quote.

Offer valid until August 31st, 2018.
Cannot be combined with other offers or discounts.
Free item must be of equal or lesser value.  All items must be on the same order.
Promotion Code US0618. 

 

 

Mirus Bio – Extra 15% OFF Introductory Pricing for LabelIT® MFP488 Nucleic Acid Labeling Reagent

Special Introductory Pricing ends June 30th, 2018.  For the months of May and June, we are offering an additional 15% OFF this introductory pricing to make it even easier for you to try this new product in your lab!

  • High Photostability – Facilitates longer time for observation and image capture
  • Widespread Instrument Compatibility – Spectrally similar to fluorescein
  • Stable at Low pH – Fluorescence is maintained over a broad range (pH 4-9)

 

Click here for more information on LabelIT® Products
Click here to order or request a quote.

Offer valid until June 30th, 2018.
Cannot be combined with other offers or discounts.
Promotion code MIR488.

 

Vector Laboratories - Buy a Vector® TrueVIEW™ Autofluorescence Quenching Kit and Get 50% OFF a VECTASHIELD® Antifade Mounting Medium

Vector® TrueVIEW™ Quencher uses a unique, patent-pending quenching agent to specifically bind and quench autofluorescent elements from non-lipofuscin sources, even in the most problematic tissues.

VECTASHIELD® Antifade Mounting Media formulations offer unsurpassed protection against fading and photobleaching, thereby, providing excellent signal retention for image acquisition and specimen archiving.

 

Click here for more information on Vector® TrueVIEW™
Click here for more information on VECTASHIELD®
Click here to order or request a quote.

Offer valid until June 30th, 2018.
Cannot be combined with other offers or discounts.
Promotion Code VHS18.

 

 

Micronic – Buy One, Get One Specials on Cappers, Tubes and Scanners

These offers will help improve your workflow by automating the process of (de)capping and scanning your sample storage tubes.

 

Buy One Manual Capper CM480, Get One at 50% OFF

The CM480 Capper offers a cost effective way of applying uniform pressure to push caps in an entire 96 tubes rack in only a single action.

Click here to order.

 

 

 

 

 

Buy One Case of External Thread Tubes, Get One Wireless Scanner for FREE

The small lightweight scanner is practical to carry around, easy-to-use and can read any 1D barcode or 2D Data-Matrix code on 24-, 48- and 96-well format tubes and racks. 

Click here to order.

Offers Valid until June 30th, 2018.
Cannot be combined with other offers or discounts.
Promotion Code MIC311

 

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TECHNICAL REPORTS

 

Vector Laboratories – Immunohistochemistry Troubleshooting Tip

I am performing IHC on tissue sections using a mouse IgG primary antibody.  My detection system is a biotinylated anti-mouse IgG secondary antibody, a VECTASTAIN® Elite ABC peroxidase kit (VECTPK6100), in combination with a DAB substrate kit (VECTSK4100), and I do not see any staining. How do I know if the end detection reagents are working?

Make a fresh working solution of DAB substrate per instructions.  Place a small volume (~1 mL) of this DAB substrate into a clean glass test tube.  To this 1 mL, add one drop (~50 µl) of Reagent B only from the VECTASTAIN® Elite ABC kit.  If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed.  This indicates the end detection reagents are working appropriately.

 

Contact us for more information on Vector IHC Products and Tips.

 

Associates of Cape Cod - Endotoxin Test Concerns of Biologics Part II: Developing New Tools

American Pharmaceutical Review™ article re-publication.

Bacterial Endotoxin Test (BET) users seek ways to overcome low endotoxin recovery (LER) from direct spikes into undiluted biologics.  These studies have come about from Chen’s initial observation that Control Standard Endotoxin (CSE) spikes, when placed into undiluted biologics, buffers, and other constituents often cannot be recovered.  By changing the endotoxin spike requirement from diluted product to undiluted product, users will unsurprisingly encounter test interference; what is unexpected from Chen’s LER finding is that recovery sometimes cannot be improved by dilution. This is indicative of a still undefined binding phenomenon.  Chen originally dismissed protein binding as a mechanism of LER as placebo with no protein of the same formulation also exhibited a low endotoxin spike recovery problem.  However, we should not assume that the binding phenomenon with protein and without protein demonstrate the same mechanism of spike loss.  This article will discuss a couple of different phenomena to explain LER observations; they may be dependent on both the protein concentration and the protein charge (anionic versus cationic) as well as surfactant and excipient effects.

 

Click here to read more.

* PDA Conference presentation by Joseph Chen, Genentech, 2013

 

NuGEN - Simple, Accurate, Molar Quantification of NGS Libraries

Accurate quantification of NGS libraries is required for proper loading of sequencing instruments and uniform read distribution across multiple samples.  Mass-based measurements such as UV absorbance or intercalating fluorescent dyes are not sufficient, since proper loading requires knowing the molar library concentration.  Users must estimate the average size of the library in order to calculate the molar library concentration.  This not only requires a separate fragment size analysis to be performed but also contributes to uncertainty especially in libraries with broader insert length distributions.  Here, NuGEN describes the performance of a novel library quantification method that produces a molar library concentration without the need for a separate size analysis.  The method can be performed with a simple fluorescent measurement, such as can be obtained with a standard fluorescent plate reader or Qubit instrument.  Excellent linearity was observed across a range of input concentrations, limited only by the dynamic range of the fluorescent reader.  NuGEN tested the method against a range of different average library sizes and size distributions and found good agreement with a popular real-time PCR based quantification method.  More importantly, they found that the method correlates well with cluster density on Illumina sequencers.

 

Click here to read more.

 

Mirus Bio - DNA Transfection Optimization Protocol

This protocol offers step-by-step instructions for a Reagent-to-DNA titration designed to determine the optimal DNA transfection conditions for any cell type using Mirus Bio Broad Spectrum and Cell Type Specific Reagents.

 

Click here to read more. 
Contact us for more information or to request a sample.

 

Hycult Biotech – Antimicrobial Peptides in Obstructive Uropathy

Antimicrobial peptides (AMPs) have historically been evaluated for their role in protecting against uropathogens. However, AMPs have not been evaluated for expression in noninfectious urological injury such as urinary tract obstruction. The objective of this pilot study is to compare urinary AMP expression in children undergoing surgical intervention for ureteropelvic junction obstruction (UPJO) with non-obstructed controls.

 

Click here to read more.  (Link to Hycult Biotech website)

 

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FEATURED PRODUCT FROM OUR SISTER COMPANY
CHROMATOGRAPHIC SPECIALITES

 

Bio-Chem Fluidics  - Solenoid Valves for Isolation and Flow Selection in Analytical Chemistry and Life Sciences

Ideal for custom built lab equipment or maintaining commercial instruments.  If your instrument’s maker no longer supplies a custom Bio-Chem valve for repairs, give us a call.

  • Isolation Valves available in Normally Open, Normally Closed or 3-way configurations; turn on, shut off, or switch a single flow path in milliseconds
  • Flow Selection Valves allow from 2 to 8 flow paths to be selected independently
    • Compact design for solvent / reagent selection
    • Easy to install, no extra tubing required
    • Factory matched flow rates for each channel ensures consistent delivery between paths
    • All flow paths of equal length (unlike linear manifold systems)

All these valves feature:

  • Inert fluid path
    • All wetted parts are inert polymers, no metallic wetted metallic parts
  • Bi-directional operation
    • Isolation valves identify the preferred inlet port for convenience, but run equally well in both directions
    • Flow selection valves can be used as common inlet (flow distribution) or common outlet (flow mixing) for 2 to 8 inlet or outlet streams.
  • Fast response times
    • Switching time from port-to-port is generally less than 20ms
    • Small overall size also helps keep the internal volumes minimized

 

Click here for more information on isolation valves. 
Click here for more information on 2 to 8 stream flow selection valves. 
Click here to order or request a quote. 

 

Bio-Chem Fluidics products are available to us through our Sister Company, Chromatographic Specialties Inc.
To simplify your purchasing process, Chromatographic Specialties Products can be purchased through MJSBioLynx, and vice versa.

 

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MJSBioLynx WELL BEING

 

How to Take Your Meditation Outdoors for People Who Don't Really Want to Sit Still

"This article features tips and ideas for meditating outdoors.  This is ideal for people who find it difficult to adjust to the silence and physical inactivity required for most types of meditation.  Tapping into a meditative state outside of the traditional methods of meditation is possible—whether running, biking, or swimming. Plus, some extra time in nature is always good for the mind and body."

 

Click here to read more.  (Link to mindbodygreen website)
Click here to visit the MJSBioLynx Well Being website.

 

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