May 2016
Dear Researcher,
We are pleased to present the e-Lynx, a newsletter devoted to keeping our customers up-to-date on new technologies, our new products, suppliers, activities and promotions.
- New Products
- New Resources
- Promotions
- Technical Report
- MJSBioLynx Well Being
NEW PRODUCTS
Micronic – Univo Screw Cap Recapper SR096
- Caps or decaps 96 screw cap tubes at once in less than 30 seconds
- Compatible with the complete Micronic range of 96-format tubes (up to 1.40mL)
- Precise torque value for optimal sealing quality
- No adapters required
- Easy-to-use graphical touch screen interface for labware selection
Click here for more information.
Click here for the video.
Click here to order or request a quote.
Affymetrix USB – Economical USB Taq DNA Polymerase
- No detectable contaminating endonucleases or exonucleases, which offers consistent performance and ensures quality results
- A highly processive 5' → 3' polymerase activity and a 5' → 3' exonuclease activity
- Available in economical pack sizes to meet a range of demands
- Supplied with 10X PCR Reaction Buffer
Click here for more information.
Click here to order or request a quote.
Mirus – Phosphatase and Protease Inhibitors
Phosphatase Inhibitor Cocktail
For inhibition of serine/threonine and protein tyrosine phosphatases
- Convenient – Optimized formulation suitable for cell and tissue extracts
- High Activity – Effectively protects phosphoproteins from phosphatases present in cell lysates
- Broad Spectrum – Inhibits serine/threonine phosphatases and protein tyrosine phosphatases
Protease Inhibitor Cocktail
For mammalian cell and tissue extracts
- Convenient – Ready-to-use solution
- High Activity – Optimized formulation for mammalian cell extracts
- Broad Spectrum – Provides complete protection during protein isolation
- Compatible – Available in animal-origin-free format
Click here to order or request a quote.
Anatrace – Trehalose Based Detergents
These detergents are based on the molecular backbone of DDM, but the different sugar head group enables them to outperform DDM 2:1 in thermal stability assays, while performing equally as well in solubilization studies.
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Click here to order or request a quote.
Simport – T-Sue™ Tissue Microarray Mold Kits
- Process up to 170 specimens onto one slide
- Easily stored in a drawer
- Can be reused hundreds of times without losing its flexibility
- Withstands temperatures from -100 °C to +250 °C
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Click here to order or request a quote.
NEW RESOURCES
NuGEN – Selection Chart for NGS, Microarray and qPCR Sample Prep / Workflow Solutions
NuGEN products enable customers to capture the truest biology achievable, independent of sample quantity or quality, using efficient sample preparation/workflow solutions for decreased time to result and increased sample value.
Click here to find the best product for your application.
PROMOTIONS
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Exclusive Promotion: Hycult Biotech – Buy 1, Get 1 FREE – Innate Immunity Research Tools
Hycult Biotech offers antibodies, proteins and immunoassays for innate immunity research, including complement, neutrophil proteins, TLR, scavenger receptors and acute phase proteins.
Click here for more information.
Click here to order or request a quote.
Click here to ask us a question.
Reference Promo Code HYB0516X at time of order.
Offer valid until May 31st, 2016.
Free item must be of lesser or equal value than item purchased
Cannot be combined with other offers or discounts.
Micronic – Special Offers on Capping and Decapping Solutions
Save on equipment for capping and decapping TPE Push Caps and Screw Caps. These solutions are a great way to speed up your sample storage workflow!
Push Cap Capping and Decapping Solution
Get a Univo Electric Decapper DC480 with a FREE Univo Electric Capper CP480
Product Number MSPROMO1604
Click here to order
Screw Cap Recapping Solution
Save 20% OFF the Univo Screw Cap Recapper SR008
Product Number MSPROMO1605
Click here to order.
High quality and a high level service are of paramount importance at Micronic.
The equipment is available with a 3-year warranty.
Offer valid until June 30th, 2016.
Cannot be combined with other offers or discounts.
Try the New HT-ExoSAP-IT® Fast One-Step PCR Clean-Up
- Half the time of standard protocols
- 100% recovery and only 5 µL of PCR product needed
- One simple pipetting step
Formulated and formatted for automated platforms and multi-channel pipettes
Click here to request a Sample.
Click here for more information.
Click here to order or request a quote.
Affymetrix USB - Take the ExoSAP-IT® PCR Clean-up Challenge
See why ExoSAP-IT reagent comes out on top.
- Request a free sample
- Do your own head-to-head comparison
- Give us your feedback for a free T-shirt
- One-tube/one-step PCR clean-up – Add Affymetrix USB ExoSAP-IT® reagent directly to PCR product
- Conserve PCR samples – 100% recovery of both short and long PCR products for accurate sequencing results
- Green option – Generate less waste with our single-tube solution to PCR purification vs. columns
- Eliminate spin columns – Decrease time and expense while increasing yield
Click here to get started.
TECHNICAL REPORT
Optimization of DNA, RNA and RNP Delivery for Efficient Mammalian Cell Engineering using CRISPR/Cas9 – by Mirus
The CRISPR/Cas9 genome-editing platform is a versatile and powerful technology to efficiently create genetically engineered living cells and organisms. This system requires a complex of Cas9 endonuclease protein with a gene-targeting guide RNA (gRNA) to introduce double-strand DNA breaks (DSBs) at specific locations in the genome. The cell then repairs the resulting DSBs using either homology-directed repair (HDR) or the error prone non-homologous end joining (NHEJ) pathway. Both DNA repair pathways can be leveraged in different ways to introduce desired modifications at the target locus.
The success of CRISPR genome editing experiments is limited by the intracellular delivery and expression of Cas9 protein and gRNA. Many methods for achieving Cas9-mediated cleavage have been identified, and the choice of DNA, RNA or ribonucleoprotein (RNP) format is dictated by experimental goal and cell type. Transfection of each type of molecule requires specific considerations for efficient functional delivery. We performed transfections using different combinations of molecules including: plasmid DNA, messenger RNA, Cas9 protein and gRNA to maximize targeting of the Cyclophilin B (PPIB) gene in HEK 293T/17, U2OS, and other mammalian cell types. Our results extend the utility of the CRISPR/Cas9 system by identifying optimal transfection conditions for intracellular delivery of Cas9 and gRNA in different formats. Click here to read more.
MJSBioLynx WELL BEING
The Neuroscience of Mindfulness Meditation - by Sarah McKay
Devotees of mindfulness meditation are eager to share the many benefits, including relaxation, positive shifts in mood, and more focused attention. But what's the scientific evidence behind how it affects our physical state of being? Keep reading to get a view into the latest neuroscience research findings on the positive health benefits of a meditation practice. Visit http://www.chopra.com/ccl/the-neuroscience...