Protein A ELISA kit
- Supplier: Cygnus Technologies
Product Description
- CYGF400 incorporates some improvements over CYGF050 and CYGF050H kits and is designed to better detect newer, unnatural constructs of Protein A, such as the MabSelect SuReTM ligand sold by GE Healthcare.
- If using a recombinant form of Protein A with very significant structural differences from natural Protein A such as MabSelect SuReTM CYGF400 is suggested. CYGF400Z is designed to overcome low analytical recovery of Protein A encountered in many commercial kits. Most low recovery samples are either incomplete antibody fragments or so called fusion proteins where the antibody structure is linked to another non-antibody molecule. The properties of such unnatural drug substance constructs means that conventional sample treatment procedures are ineffective in dissociation, denaturation, precipitation and removal of the drug substances prior to using the Protein A detection assay. Any un-denatured drug substance remaining in the sample after the treatments step can re-associate with leached Protein A, resulting in under recovery by ELISA.
- CYG400Z has been qualified using several problematic samples and found to yield acceptable recovery on all.
- CYG400Z uses the same antibodies standards and sample treatment protocol as CYGF400 except that it also contains a "Recovery Enhancer" solution, which is combined with HRP conjugated anti-Protein A antibody prior to use.
- "Recovery Enhancer" prevents re-association of any drug substance that was not completely removed during the sample treatment step.
- CYGF400Z will accurately quantitate the unnatural construct of Protein A such as the MabSelect SuReTM as well as other conserved, natural and recombinant Protein A constructs. Because not all drug substances will respond equally to sample treatment procedures, each new sample type and drug substance must be validated for acceptable analytical recovery before reporting results.
- Since released, 2 newer Protein A assays (Mix-N-Go methods) have been developed. These eliminate sample boiling and centrifugation steps. Instead, a special sample treatment reagent is used that allows for accurate detection of Protein A in the presence of the sample antibody.
- CYGF600 kit uses standards calibrated against natural and conserved recombinant forms of Protein A.
- CYGF610 kit uses standards calibrated to unnatural constructs of Protein A such as GE Healthcare's MabSelect SuReTM.
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Product Options
| Product Number | Description | Package Size | List Price (CAD) | ||
|---|---|---|---|---|---|
| CYGF400 | Protein A ELISA kit | 1 kit | -- | Add to Cart | |
| CYGF400Z | Protein A ELISA Kit | 1 kit | -- | Add to Cart |
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