Cygnus Technologies

CHO HCP ELISA kit, 3G

Product Description

  • 3rd Generation ELISA kit uses a new and even more broadly reactive antibody than earlier kits.
  • Incorporates a number of other improvements in the detection of CHO HCPs.
  • More sensitive than previous kits, able to detect HCPs in the range of 100 parts per billion for a variety of antibodies and other therapeutic proteins expressed in CHO.
  • Extensively qualified with a large number of drug substances and in-process samples.
  • Generic method that can likely be validated as not only a process development tool, but in most cases as a lot release test, eliminating the need to develop a custom or process specific assay.
  • Antibody has been evaluated for reactivity to individual HCPs using the conventional 2D western blot as well as a method we have shown to be superior to 2D WB (2D HPLC-ELISA). 2D HPLC-ELISA method has demonstrated that the antibody is reactive to more than 750 individual HCPs from conditioned media and cell lysates in both a CHO-S strain as well as a K1 strain. These 750+ HCPs represent more than 98% of the total mass of protein, as indicated by protein detection methods orthogonal to ELISA. Thus, this antibody may overcome concerns about missing any significant individual HCPs.

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Product Number Description Package Size List Price (CAD)
CYGF550 CHO HCP ELISA Kit, 3G 1 kit
CA$1,472.00
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