CUTANA™ Quick Cleanup DNA Purification Kit
- Supplier: EpiCypher
- Storage Conditions: Store at room temperature. Stable for 1 year upon date of receipt.
Product Description
- The CUTANA™ Quick Cleanup DNA Purification Kit can be used to purify CUT&RUN or CUT&Tag DNA and remove unwanted adapter-/primer-dimers from these reactions. Unlike many kits that purify DNA using spin columns, this kit uses SPRI paramagnetic beads for streamlined, higher throughput workflows easily integrated into CUTANA™ genomic mapping assays. Depending on the application, various SPRI bead:DNA ratios are added to capture target DNA.
- In CUT&RUN, pAG-MNase cleaves DNA proximal to antibody-labeled chromatin, releasing it into the supernatant. It is imperative to recover the highest DNA yield possible from the supernatant; therefore, a higher bead:DNA ratio is added to span a wide range of DNA fragment lengths. This kit includes sufficient volume to perform 48 total CUT&RUN reactions at an empirically optimized higher bead ratio.
- In CUT&Tag, pAG-Tn5 cleaves antibody-bound chromatin and simultaneously ligates adapter DNA, resulting in longer DNA fragments. Further, in the EpiCypher CUTANA™ Direct-to-PCR CUT&Tag workflow, indexing PCR is used to amplify next-generation sequencing (NGS) libraries directly from the reaction mixture prior to DNA purification. In this case, a lower bead ratio is ideal for preferable enrichment of longer DNA fragments and selective cleanup of sequencing libraries. This kit includes sufficient volume to perform 96 total CUT&Tag reactions at an empirically optimized lower bead ratio.
Resources
Product Options
| Product Number | Description | Package Size | List Price (CAD) | ||
|---|---|---|---|---|---|
| EP140052 | CUTANA™ Quick Cleanup DNA Purification Kit | 48 CUT&RUN -OR- 96 CUT&Tag Reactions | -- | Add to Cart |
List Prices are displayed.
Please register or log-in to the site to view your organization’s prices, or please add the products to your cart and click the “Request a Quote” button to receive price and availability information (include quantities for each product and indicate either a one-time purchase or your annual usage.)