Mononucleosomes, Recombinant Human, Desthiobiotinylated
- Supplier: EpiCypher
- Storage Conditions: Mononucleosomes, Human Recombinant Desthiobiotinylated are highly purified and suitable for use as substrates in enzyme screening assays, structural studies, or effector protein binding experiments. The weaker affinity of desthiobiotin for avidin-family proteins allows nucleosomes to be eluted via addition of free biotin. The ability for these nucleosomes to be recovered intact is useful in many aspects of chromatin biology.
Product Description
- Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.1, and H4 Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5™ desthiobiotin-TEG group. Desthiobiotin has a weaker affinity (Kd = 10-11) for avidin-family proteins than does biotin (Kd = 10-15).
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Product Options
| Product Number | Description | Package Size | List Price (CAD) | ||
|---|---|---|---|---|---|
| EP160024 | Mononucleosomes, Recombinant Human, Desthiobiotinylated | 50 µg |
CA$779.00
|
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