Acidic Patch Mutant H2AE92K Biotinylated
- Supplier: EpiCypher
- Storage Conditions: Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
- Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A*, H2B, H3 and H4; accession numbers: H2A-P04908*; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA  containing a 5’ biotin-TEG group.
- *Histone H2A contains a glutamate-to-lysine (E-to-K) substitution at position 92 (H2AE92K). H2AE92 is among key residues forming a negatively charged region on the nucleosome surface named the “acidic patch”. The acidic patch is a conserved interaction hub for neighboring nucleosomes and nucleosome binding proteins, often via salt bridges with arginine anchors, and is functionally critical in chromatin condensation and chromatin remodeling [2-4]. H2AE92 resides in the H2A C-terminal extension and is associated with nucleosome binding factors such as histone H4 N-terminal tail, LANA, RCC1, IL-33, SIR3, HMGN2, and remodeling ATPases [2-4]. H2AE92K disrupts binding with SMARCB1 .
|Product Number||Description||Package Size||List Price (CAD)|
|EP160030||Nucleosome, Recombinant Human, Acidic Patch Mutant H2AE92K Biotinylated||50 µg||
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