Nucleosome, Recombinant Human, H3K14ub1 dNuc, Biotinylated
- Supplier: EpiCypher
- Storage Conditions: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
Product Description
- Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains ubiquitin-lysine at position 14. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group. *H3K14ub1 has a Cys to Ala substitution at position 110.
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Product Options
| Product Number | Description | Package Size | List Price (CAD) | ||
|---|---|---|---|---|---|
| EP160398 | Nucleosome, Recombinant Human, H3K14ub1 dNuc, Biotinylated | 25 µg |
CA$861.00
|
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