HOOK™ BiotinQuant Kit
- Supplier: G Biosciences
- Storage Conditions: Room Temperature
Product Description
- A kit for estimation of biotin conjugation
- Biotin labeling is measured using HABA [4'-hydroxyazobenzene-2-carboxylic acid] dye.
- HABA binds with avidin at the biotin binding site.
- The HABAavidin complex produces a characteristic color that absorbs at 500nm. (ε = 34,000 M-1 cm-1 expressed as per mole of HABA bound).
- In the presence of biotin or biotinylated agents, these agents compete with the HABA for the binding sites.
- Since biotins have a greater affinity for the binding sites it results in the displacement of HABA from the avidin binding sites and proportionate reduction of absorbance.
- Hence, the amount of biotin present in the solution can be determined by measuring the reduction in absorbance at 500nm.
Resources
Product Options
| Product Number | Description | Package Size | List Price (CAD) | ||
|---|---|---|---|---|---|
| GBBKC01 | HOOK™ BiotinQuant Kit | 20 Assays |
CA$335.80
|
Add to Cart |
List Prices are displayed.
Please register or log-in to the site to view your organization’s prices, or please add the products to your cart and click the “Request a Quote” button to receive price and availability information (include quantities for each product and indicate either a one-time purchase or your annual usage.)