Product Description

• The Human S100A7 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours.
• The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay.
• Samples and standards are incubated in microtiter wells coated with antibodies recognizing Human S100A7.
• Biotinylated tracer antibody will bind to the captured Human S100A7.
• Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody.
• Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB).
• The enzyme reaction is stopped by the addition of oxalic acid.
• The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the Human S100A7 standards (log).
• The Human S100A7 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.