Vector Laboratories

SNA, EBL, Agarose

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Product Description

  • Sambucus nigra lectin SNA, EBL, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in alpha-2,6 and to a lesser degree, alpha-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalactosamine. SNA has been reported to inhibit cell-free protein synthesis.Agarose bound* Sambucus nigra lectin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.This coupling method provides several advantages over the traditional cyanogen bromide procedure:Maximum carbohydrate binding activity of the coupled lectins is retainedLinkage is stable over a range of pH valuesConjugated proteins are not leached off the beads by Tris or other routinely used buffersNo residual charges are present after conjugation. This minimizes non-specific binding to the matrix.Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.Elution: 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid*3 mg lectin/ml gel

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Product Options

Product Number Description Package Size List Price (CAD)
VECTAL1303 Sambucus Nigra Lectin (SNA, EBL), Agarose bound, 2 ml 2ML
CA$427.00
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