Vector Laboratories

Vector® TrueVIEW® Autofluorescence Quenching Kit with DAPI

Product Description

  • TrueVIEW Autofluorescence Quenching Kit removes unwanted autofluorescence in tissue sections due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin. Improves overall signal-to-noise in most immunofluorescence assays. Effective on problematic tissues such as kidney and spleenEasy-to-use, one-step method. Compatible with a wide selection of fluorophores. Compatible with standard epifluorescence and confocal laser microscopes. Tissue autofluorescence often occurs with aldehyde fixation or from inherent native tissue components (collagen, elastin, and red blood cells). The extent and intensity of autofluorescence background frequently makes it difficult or impossible to distinguish specific signals in immunofluorescence applications.Most methods for reduction of tissue autofluorescence act primarily on lipofuscin granules, and are not broadly effective against the most common sources of autofluorescence targeted by TrueVIEW Quencher. TrueVIEW Autofluorescence Quenching Kit provides a novel way to diminish unwanted autofluorescence from non-lipofuscin sources and dramatically improve signal-to-noise ratio. The quenching action of the kit reagents provides a clear, unambiguous, "true view" localization of the target antigen.Current methods for reducing autofluorescence primarily include "home brew" concoctions such as sodium borohydride and other ink-based products. These methods are essentially ineffective against aldehyde induced autofluorescence. In contrast, TrueVIEW reagent binds to hydrophilic compounds and effectively quenches endogenous autofluorescence. Kit Contents:TrueVIEW Reagent A 5 ml, TrueVIEW Reagent B 5 ml, TrueVIEW Reagent C 5 ml, VECTASHIELD Vibrance Antifade Mounting Medium 2 ml. One kit is sufficient to treat approximately 100 to 150 tissue sections.


Product Options

Product Number Description Package Size List Price (CAD)
VECTSP8500 Vector® TrueVIEW® Autofluorescence Quenching Kit with DAPI, 1 kit 15ML
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