Sunrise Science Products - Yeast Media

  • Premixed media offers superior reproducibility
  • Save time and expense of weighing individual powders
  • Exceptional variety for research flexibility
  • Manufactured with high-quality raw materials

 

 

PRODUCTS

 

 

Yeast Media Building Tool
Use the Build Media Tool on the Sunrise website to modify an existing media formula, or build your own from scratch. 
Click here for the Build Media Tool (Link to Sunrise Website)

 

Custom Products
Sunrise Science Products' commitment to customization makes it easy to change a formulation, remove sugars, nitrogen, amino acids, salts, metals or anything else. 
Contact us for more information. 
 

 

TECHNICAL INFORMATION

 

How is Defined Media Defined?

Sunrise Science Products starts with Yeast Nitrogen Base (YNB). In their terminology, YNB is the base to which ammonium sulfate or another nitrogen source is added. YNB contains salts, vitamins, and trace elements.

Upon the addition of ammonium sulfate, YNB becomes “YNB+Nitrogen”. Variations of YNB and YNB+Nitrogen are available that are missing specific components as identified by a minus sign in the product name.

Adding glucose to YNB+Nitrogen creates a dropout base (DOB). If agar is added at this point, it is a dropout base with agar (DOBA).  They also offer DOB/DOBA formulations with Raffinose, Galactose, or both, which will be clear from the product name.

An amino acid mixture can then be chosen based on the concentrations and components of the CSM, SC, HSM or BSM formulations (see table 2). When a CSM amino acid mix is added to either DOB or DOBA, it becomes a complete defined media (SD or SDA). When an SC amino acid mix is added to DOB, it becomes an SC Complete.

 

  Contains
  YNB
 		 Nitrogen
 		 Sugar
 		 Amino Acid
Mix		 Agar
 
SD x x x x (CSM)  
SDA x x x x (CSM) x
SC Complete x x x x (SC)    
DOB x x x    
DOBA x x x   x
YNB x        
YNB + Nitrogen x x      
CSM       x  
SC       x  
HSM       x  
BSM       x  

Yeast Nitrogen Base (YNB) Contains: Potassium phosphate (1000 mg/L), Magnesium sulfate (500 mg/L), Sodium chloride (100 mg/L), Calcium Chloride (100 mg/L), Biotin (0.002 mg/L), Pantothenate, calcium (0.4 mg/L), Folic acid (0.002mg/L), Inositol (2 mg/L), Niacin (0.4 mg/L), PABA (0.2 mg/L), Pyridoxine (0.4 mg/L), Riboflavin (0.2 mg/L), Thiamine (0.4 mg/L), Boric acid (0.5 mg/L), Copper sulfate (0.04 mg/L), Potassium iodide (0.1 mg/L), Iron (III) chloride (0.2 mg/L), Manganese sulfate (0.4 mg/L), Sodium molybdate (0.2 mg/L), Zinc sulfate (0.4 mg/L).

 

Table 2: Components of Amino Acid Mixtures

  Amount in each mixture (mg/L)
Component CSM SC BSM HSM SP
4-Aminobenzoic acid (PABA)  0.0  8.6  0.0  0.0  0.0
Adenine hemisulfate*  10.0  21.0  40.0  100.0  50.0
Inositol  0.0  85.6  0.0  0.0  0.0
L-Alanine  0.0  85.6  0.0  0.0  0.0
L-Arginine  50.0  85.6  20.0  100.0  0.0
L-Asparagine monohydrate  0.0  85.6  0.0  0.0  0.0
L-Aspartic acid  80.0  85.6  100.0  50.0  0.0
L-Cysteine hydrochloride monohydrate    0.0  85.6  0.0  100.0  0.0
L-Glutamic acid  0.0  85.6  100.0  0.0  0.0
L-Glutamine  0.0  85.6  0.0  0.0  0.0
L-Glycine  0.0  85.6  0.0  0.0  0.0
L-Histidine hydrochloride monohydrate  20.0  85.6  20.0  50.0  50.0
L-Isoleucine  50.0  85.6  300.0  50.0  0.0
L-Leucine  100.0  173.4  60.0  100.0  50.0
L-Lysine hydrochloride  50.0  85.6  30.0  100.0  50.0
L-Methionine**  20.0  85.6  20.0  50.0  0.0
L-Phenylalanine  50.0  85.6  50.0  50.0  0.0
L-Proline  0.0  85.6  0.0  50.0  0.0
L-Serine  0.0  85.6  375.0  50.0  0.0
L-Threonine  100.0  85.6  200.0  100.0  0.0
L-Tryptophan  50.0  85.6  40.0  100.0  0.0
L-Tyrosine  50.0  85.6  30.0  50.0  0.0
L-Valine  140.0  85.6  150.0  50.0  0.0
Uracil  20.0  85.6  20.0  100.0  50.0

*Minimum quantity for healthy growth and yet optimized to promote red color in certain adenine auxotrophs. Additional CSM formulations are available that contain different amounts of adenine.
**Due to their relationship to methionine biosynthesis, threonine is replaced by homoserine in mixtures lacking methionine.

References:

Complete Supplement Mixture (CSM): The formulation for CSM has been time-tested as a dropout supplement for virtually all strains of S. cerevisiae.

Synthetic Complete Mixture (SC): SC is also known as Hopkins Mixture. [F.Spencer et.al. Targeted recombination-based cloning and manipulation of large DNA segments in yeast. Methods: A companion to methods in enzymology 1993; 5:161-175].

Brent Supplement Mixture (BSM): For use with the Interaction Trap two-hybrid system. [Gyruis, J., et.al. 1993. Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2. Cell 75:791–803].

Hollenberg Supplement Mixture (HSM): For use with Hollenberg’s modification of Field’s two-hybrid system for HIS selection. Use HSM in conjunction with DOB w/Succinate. [Hollenberg, S.M., et.al. 1995. Identification of a New Family of Tissue-Specific Basic Helix-Loop-Helix Proteins with a Two-Hybrid System. Mol. and Cell Biol. 15, 3813].