NuGEN - Unique Indexing Solutions for Illumina’s High Capacity Sequencing Platforms
NuGEN’s Unique Dual Indexes are designed to enable researchers to take full advantage of the latest generation of Illumina sequencers. They detect index hopping, a sequencing phenomenon that can lead to mis-assignment of reads to the incorrect sample. Use of an additional index can enable the detection and eliminate mis-assigned reads from a dataset. This is particularly critical in applications that require high sensitivity, and improves accuracy of sample assignment in any experiment. Index hopping has been shown to occur at a higher frequency in patterned flow cell sequencing platforms, such as the HiSeq 3000, HiSeq 4000, NovaSeq.
The Unique Dual Indexes are conveniently configured in a pre-plated format as 96 unique barcode pairs, so manual preparation of barcode pairs is completely eliminated. Simply add the adaptor to the sample and ligate, and each sample will have index1 and index2 barcodes that are completely unique from all other samples in the plate.
This feature is currently available for the Ovation® Ultralow System V2 for DNA-Seq library preparation, and for the Universal Plus mRNA-Seq and Trio RNA-Seq system for preparation of RNA-Seq libraries. Solutions for the Methyl-Seq product line will also be available soon.
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